| Synthesis of functionalized Fe2O3 MNPs and determination of the drug loading efficiency of these MNPs |
| کد مقاله : 1120-ICOC |
| نویسندگان |
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نوشین بیجاری * Department of Biology, Faculty of Basic Sciences, Semnan University, Semnan, Iran |
| چکیده مقاله |
| Abstract: Objectives: Magnetic nanoparticles (MNPs) represent one of the most promising nanomaterials for biological applications due to their biocompatibility and superparamagnetic properties [1,2]. The present study aims to synthesize functionalized MNPs with enhanced drug loading efficiency, thereby providing a versatile platform for protein purification. Methods: Fe₃O₄ magnetic nanoparticles (MNPs) were synthesized via a co-precipitation method using FeCl₃·6H₂O and FeCl₂·4H₂O [3]. Main results: The concentration of the drug loaded onto the modified Fe₂O₃ MNPs was quantified [4]. The drug solutions were prepared in the concentration range of 10.0–18.0 µg/mL. The absorbance of each solution was recorded at 257 nm, corresponding to the maximum absorption peak of the drug, and a calibration curve of absorbance versus drug concentration was constructed. The regression equation was determined as y = 15,930x + 0.0043. This equation was subsequently employed to calculate the drug content in the modified Fe₂O₃ MNPs. The drug loading efficiency (R%) in modified Fe2O3 MNPs was calculated using following Equation: R% = (Wdrug/Wnanoparticle) × 100 (1) where, Wdrug and Wnanoparticle represent the weight of drug in the MNPs and weight of the MNPs, respectively. The loading efficacy of the nanoparticles was attained as 49.4%. Conclusions: The MNPs comprising drug-conjugated γ– Fe2O3–3- chloropropyltriethoxysilane MNPs were created for protein purification. The 3- chloropropyltriethoxysilane coating was used for stabilizing Fe3O4 nanoparticles and also as a linker for drug, where drug was able to bind to protein specifically. |
| کلیدواژه ها |
| Magnetic nanoparticles (MNPs), Drug loading efficiency, Protein |
| وضعیت: پذیرفته شده |