| Peroxidase-like Catalytic Activity of a MOF-Based Composite for Biochemical Applications |
| کد مقاله : 1337-ICOC |
| نویسندگان |
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مرضیه فرخنده نیا * دانشجو |
| چکیده مقاله |
| Metal-organic frameworks (MOFs) are crystalline materials with a porous structures, consisting of metal ions coordinated with multifunctional organic linkers. These structures are well-defined and offer tunable pore sizes, surface areas, and chemical properties [1]. These characteristics render MOFs suitable platforms for applications in catalysis, adsorption, sensing, and enzyme mimicry [2]. This study presents the synthesis of a Cu-MOF composite using 2-aminoterephthalic acid (NH₂-BDC) as the organic linker, which was designed to provide a stable framework with accessible active sites for catalysis. The synthesized Cu-MOF was characterized utulizing several methods, and its enzyme-mimicking activity was evaluated using TMB and H₂O₂ as substrates. Catalytic studies were conducted under varying pH (3.0 to 9.0) and temperature (25-80 °C) conditions. The MOF exhibited maximal catalytic activity at a pH of 6.0 and 45 °C, indicating the strong dependence of its peroxidase-like activity on environmental conditions. The kinetic parameters of Cu-MOF were further investigated using steady-state kinetics, which were performed under optimal conditions.The Michaelis−Menten constant (Km) and maximum initial velocity (Vmax) were calculated from a Lineweaver−Burk plot. The Kₘ values of Cu-MOF for H₂O₂ and TMB were determined to be 23.20 mM and 6.30 mM, respectively, while the corresponding Vₘₐₓ values were 0.4678 ×10⁻³ M•s⁻¹and 0.234 ×10⁻³ M•s⁻¹, respectively. These kinetic parameters describe the catalytic performance of the MOF toward both substrates under the studied conditions. Overall, the Cu-MOF composite exhibited a structurally robust and morphologically uniform framework, demonstrating significant peroxidase-like activity. This emphasizes its potential as a stable nanozyme platform for various biochemical and catalytic applications. |
| کلیدواژه ها |
| Keywords: Metal-Organic-Farameworks, Peroxidase-like activity, Hybrid nanostructures |
| وضعیت: پذیرفته شده |