سی و یکمین کنفرانس شیمی آلی انجمن شیمی ایران

Study of Alpha-Pinene Release from Lipid Nanoparticles and Its Effect on Breast Cancer

کد مقاله : 1376-ICOC

نویسندگان

رضوان پارسائی *¹، سید علی حسینی²، یعقوب پاژنگ²

¹دانشجو

²دانشگاه ارومیه

چکیده مقاله

Abstract

In this study, the release of alpha-pinene capsulated by a solid lipid nanoparticles (SLN) carrier was studied in-vitro medium. The study was done at different pH, i.e. 54. And 7.4. The results showed that the drug release from SLN at 7.4 is better than that at 5.4 and follow a slow-releasing mode.

Introduction

Numerous studies have investigated the anticancer effects of terpenes. Specifically, 37 monoterpenes from essential oils of plants have shown antitumor activity.Anticancer studies of alpha-pinene have been conducted in a variety of cancers and in vivo models[1].: Non-toxic solid lipid nanoparticles (SLN) are ideal carriers for lipophilic APIs and essential oils containing monoterpenes, and modulate the release profile of fragrances [2]. The aim of this work was the kinetic study of alpha-pinene from sln carrier and different pHs.

Experimental:Synthesis of lipid nanoparticles (SLN)

The lipid mixture (0.5% w/v oleic acid) and alpha-pinene (0.5% w/v) were dissolved and added to 1% w/v stearic acid melted at high temperature. The lipid solution was injected into an aqueous phase containing Tween 80 (1% w/v) at 70°C. The emulsion was mixed using ultrasound (150 W, 10 min) and then cooled in an ice bath. The nanoparticles were centrifuged and dried.

Results and Discussion

The release profile of alpha-pinene from lipid nanoparticles (LNPs) in phosphate buffer saline (PBS) at pH 7.4, mimicking physiological blood conditions. The cumulative drug release was monitored over time, revealing a biphasic pattern characteristic of lipid-based nanocarrier.

Conclusions

As a result, lipid nanoparticles showed controlled biphasic release of alpha-pinene at physiological pH 7.4, achieving more than 70% cumulative release within 48 hours, supporting their potential for sustained drug delivery.

References

[1] Jo, H., Cha, B., Kim, H., Brito, S., Kwak, B. M., Kim, S. T., ... & Lee, M. G. (2021). α-Pinene enhances the anticancer activity of natural killer cells via ERK/AKT pathway. International Journal of Molecular Sciences, 22(2), 656.‏

[2 Zielińska, A., Ferreira, N. R., Durazzo, A., Lucarini, M., Cicero, N., Mamouni, S. E., ... & Souto, E. B. (2019). Development and optimization of alpha-pinene-loaded solid lipid nanoparticles (SLN) using experimental factorial design and dispersion analysis. Molecules, 24(15), 2683.‏ doi:10.3390/molecules24152683.

کلیدواژه ها

Alpha-pinene, Lipid nanoparticles, Controlled release, Breast cancer, Drug delivery

وضعیت: پذیرفته شده